fibroblast cell line l929  (Procell Inc)

Journal: ACS Omega

Article Title: Comparative Study of Chitosan-Pyrophosphate and Magnesium Hydroxide-Alginate Hybrid Nanoparticles: Physicochemical Properties and Cytocompatibility toward Vascular Calcification Applications

doi: 10.1021/acsomega.5c12883

Figure Lengend Snippet: (a) Cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-Mg­(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Article Snippet: The murine fibroblast cell line L929 (ATCC NCTC clone 929 [L cell, CCL-1]) was used for cytotoxicity assays.

Techniques: Control

Journal: Bioactive Materials

Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

doi: 10.1016/j.bioactmat.2026.01.005

Figure Lengend Snippet: The biocompatibility of Ti-OH-ePV. a) Schematic illustration of the co-culture model; b) Viability of L929 cells after 24 h co-culture with Ti, Ti-OH, and Ti-OH-ePV samples; c) Live/dead fluorescence images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 200 μm); d) Cytoskeletal staining images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 100 μm); e) Hemolysis test results and f) quantitative hemolysis ratios of Triton X-100, PBS, Ti, Ti-OH, and Ti-OH-ePV groups; g) Immunohistochemical staining images of IL-4, IL-10, CD68, and IL-1β in rat subcutaneous tissues 7 days post-implantation (scale bar: 100 μm); h) Quantitative analysis of IL-4 and IL-10 expression; i) Quantitative analysis of CD68 and IL-1β expression; n = 3; ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Standard fibroblast cell line L929 fibroblasts and mouse macrophages (Raw264.7) were obtained from Jiangsu Keygen Biotech Co., Ltd. (China).

Techniques: Co-Culture Assay, Fluorescence, Staining, Immunohistochemical staining, Expressing

Journal: Bioactive Materials

Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

doi: 10.1016/j.bioactmat.2026.01.005

Figure Lengend Snippet: Evaluation of cell adhesion, proliferation, and pro-angiogenic potential of different samples. a) SEM images of Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 1 μm); b) Fluorescent images of L929 cells on Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d after co-culturing 72 h (scale bar: 200 μm); c) Fluorescent images of Matrigel tube formation by HUVECs treated with Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 200 μm); e) Scratch wound migration assay of HUVECs in the Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d groups (scale bar: 100 μm).

Article Snippet: Standard fibroblast cell line L929 fibroblasts and mouse macrophages (Raw264.7) were obtained from Jiangsu Keygen Biotech Co., Ltd. (China).

Techniques: Migration

Journal: Journal of Nanobiotechnology

Article Title: Injectable ROS-scavenging and NIR-responsive nanocomposite hydrogel for staphylococcus aureus-infected diabetic wound healing

doi: 10.1186/s12951-026-04306-4

Figure Lengend Snippet: Biological functionality and biocompatibility of the PTPM hydrogel. a ) Tube formation assay in HUVECs, demonstrating the strongest proangiogenic activity in the PTPM hydrogel group. b , c ) Quantitative analyses of branch number and total tube length further confirmed its superior vascularization potential( n = 3 per group). d ) Intracellular ROS levels in L929 fibroblasts cultured with different hydrogels revealed the most efficient ROS scavenging in the PTPM group, e , f ) supported by quantitative fluorescence intensity and area measurements. g ) Scratch-wound assays at 0 and 24 h indicated markedly enhanced cell migration with PTPM treatment( n = 3 per group), h ) corroborated by statistical analysis of migration rates( n = 3 per group). i ) Live/dead fluorescence staining of L929 cells on days 1, 3 and 5 showed excellent cytocompatibility across all groups, with the PTPM hydrogel promoting the greatest proliferation over time. j–l ) Quantitative assessments of fluorescence intensity, viability, and proliferation on days 1, 3 and 5 further verified enhanced cellular growth and biocompatibility in the PTPM group( n = 3 per group). m–p ) qPCR analysis of proinflammatory (IL-1, iNOS) and anti-inflammatory markers (IL-4, CD206) demonstrated that the PTPM hydrogel most effectively suppressed inflammatory responses and promoted an anti-inflammatory phenotype( n = 3 per group). All data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001.HUVECs, human umbilical vein endothelial cells; ROS, reactive oxygen species; qPCR, quantitative polymerase chain reaction; NS, normal saline; PVA, polyvinyl alcohol; TSPBA, N¹-(4-boronobenzyl)-N³-(4-boronophenyl)-N¹,N¹,N³,N³-tetramethylpropane-1,3-diamine; PGS, Palygorskite; MoS₂, molybdenum disulfide; PT, PVA-TSPBA hydrogel; PTM, PVA-TSPBA@MoS₂ hydrogel; PTPM, PVA-TSPBA@PGS/MoS₂ hydrogel

Article Snippet: The murine fibroblast cell line L929 (Cat. No. CL-0137) and fetal bovine serum (FBS, Cat. No. 164210) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Tube Formation Assay, Activity Assay, Cell Culture, Fluorescence, Migration, Staining, Real-time Polymerase Chain Reaction, Saline